Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography PDF Print E-mail

Han X, Zhang TY, Wei Y, Cao XL, Ito Y: Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography. Journal of Chromatography A 2002, 971(1-2):237-241.

High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% purity determined by HPLC analyses. Identification was performed by H-1 NMR, C-13 NMR, and MS.

 

Swissmedic registriertes Rhodiola Produkt: Erhältlich in Apotheken und Drogerien